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snoRNAs associate with mRNA 3' processing complex: New wine in old bottles
Junjie Shi1, Chunliu Huang1, Shanshan Huang1
1a Center for Stem Cell Biology and Tissue Engineering , Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University , Guangzhou , China.
Abstract:
3' end processing is required for the maturation of all eukaryotic RNAs. Current model suggests that canonical mRNA 3' processing is carried out exclusively within a protein complex termed mRNA 3' processing complex. In a recent study, by using RNA-biotin based pull-down assay and high-throughput sequencing, we reported that a subset of small nucleolar RNAs (snoRNAs) were physically associated with this macromolecular machinery. Through detailed characterization of one of these snoRNAs, SNORD50A, we revealed that non-coding RNA, such as snoRNA, may play a regulatory role in mRNA 3' processing. Our results provided novel insight into both the regulatory mechanism of mRNA 3' processing and the non-canonical functions of snoRNAs.