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Related Concept Videos

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Acceleration is in the direction of the change in velocity, but it is not always in the direction of motion. When an object slows down, its acceleration is opposite to the direction of its motion. Although commonly referred to as deceleration, this causes confusion in our analysis as deceleration is not a vector, and does not point to a specific direction with respect to a coordinate system. Therefore, the term deceleration is not used. For example, when a subway train slows down, it...
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Related Experiment Video

Updated: Feb 16, 2026

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
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Accelerated super-resolution imaging with FRET-PAINT.

Jongjin Lee1,2, Sangjun Park1,2, Wooyoung Kang1,2

  • 1Department of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea.

Molecular Brain
|December 30, 2017
PubMed
Summary
This summary is machine-generated.

We developed FRET-PAINT, a faster super-resolution microscopy technique. This method overcomes limitations of DNA-PAINT, enabling rapid imaging of cellular structures like microtubules and mitochondria with improved speed.

Keywords:
DNA-PAINTFRETFRET-PAINTSMLMfluorescence resonance energy transfersingle-molecule localization microscopysuper-resolution microscopy

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Related Experiment Videos

Last Updated: Feb 16, 2026

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Area of Science:

  • Biophysics
  • Microscopy
  • Cell Biology

Background:

  • Super-resolution microscopy faces challenges in imaging thick tissues due to slow speed and photobleaching.
  • DNA-PAINT reduces photobleaching but has very low imaging speed.

Purpose of the Study:

  • To develop an accelerated super-resolution fluorescence microscopy technique.
  • To overcome the speed limitations of DNA-PAINT for biological imaging.

Main Methods:

  • Developed FRET-PAINT, an accelerated super-resolution fluorescence microscopy method.
  • Utilized FRET-PAINT to image cellular structures in COS-7 cells.

Main Results:

  • Achieved up to a 30-fold increase in imaging speed compared to conventional DNA-PAINT.
  • Demonstrated super-resolution reconstruction of microtubules and mitochondria within 25-50 seconds.

Conclusions:

  • FRET-PAINT significantly enhances imaging speed for super-resolution microscopy.
  • This technique offers a viable solution for imaging neural connectivity and cellular structures in thick tissues.