Jove
Visualize
Contact Us

Related Concept Videos

Reaction Stoichiometry02:57

Reaction Stoichiometry

78.8K
A balanced chemical equation provides a great deal of information in a very succinct format. Chemical formulas provide the identities of the reactants and products involved in the chemical change, allowing classification of the reaction. Coefficients provide the relative numbers of these chemical species, allowing a quantitative assessment of the relationships between the amounts of substances consumed and produced by the reaction. These quantitative relationships are known as the reaction’s...
78.8K
Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles03:12

Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles

30.1K
Chemical stoichiometry describes the quantitative relationships between reactants and products in chemical reactions.
30.1K
Alterations in Respiration II01:30

Alterations in Respiration II

1.9K
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients.
In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes...
1.9K
RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Altered States of Awareness01:06

Altered States of Awareness

1.2K
Altered states of consciousness represent significant deviations from one's normal mental state. These deviations can range from subtle changes in awareness to profound transformations in perception, thought processes, and sensory experiences. Altered states of consciousness can be triggered by various factors, including drug use, meditation, hypnosis, illness, or even intense fatigue.
The ingestion of substances like stimulants or hallucinogens leads to chemical alterations in the brain...
1.2K
Alterations in Blood Pressure01:30

Alterations in Blood Pressure

2.2K
Alterations in blood pressure, such as hypertension (high blood pressure) and hypotension (low blood pressure), significantly affect human health. Understanding these conditions' classifications, causes, and symptoms is essential for effective management and treatment.
Hypertension (High blood pressure)
Hypertension occurs when blood pressure readings consistently exceed the normal range. It is diagnosed when systolic blood pressure (the top number, indicating pressure while the heart...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploiting conformational changes in a caffeine aptamer to engineer synthetic RNA devices.

Nucleic acids research·2026
Same author

The interaction between the RNA aptamer Dap1 and DasR mirrors DNA operator site recognition in GntR repressors.

Nucleic acids research·2026
Same author

Iterative design of a NAND hybrid riboswitch by deep batch Bayesian optimization.

Nucleic acids research·2026
Same author

Beyond the niche - unlocking the full potential of synthetic riboswitches.

Nature communications·2025
Same author

Structural basis for ligand recognition in the tobramycin riboswitch.

Nucleic acids research·2025
Same author

Triple SELEX approach for the selection of a highly specific RNA aptamer binding homoeriodictyol.

Biological chemistry·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 16, 2026

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
08:11

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

2.3K

Altered stoichiometry of an evolved RNA aptamer.

Shoji Ohuchi1, Beatrix Suess1

  • 1Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany.

RNA (New York, N.Y.)
|December 30, 2017
PubMed
Summary

Researchers enhanced inhibitory aptamer activity not by increasing binding affinity, but by altering complex formation stoichiometry. This finding offers a novel strategy for improving aptamer function in biological applications.

Keywords:
aptamerdirected evolutionrepressorstoichiometry

More Related Videos

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

3.6K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.3K

Related Experiment Videos

Last Updated: Feb 16, 2026

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
08:11

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

2.3K
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

3.6K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.3K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Synthetic Biology

Background:

  • Inhibitory aptamers are valuable tools for protein antagonism and gene repression.
  • Current strategies for aptamer improvement primarily focus on enhancing binding affinity.

Purpose of the Study:

  • To explore an alternative mechanism for improving inhibitory aptamer activity.
  • To investigate aptamer improvement beyond increased binding affinity.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) on a mutagenized aptamer pool.
  • In vivo screening and truncation of selected aptamers.
  • Biochemical analyses to determine binding characteristics and stoichiometry.

Main Results:

  • An improved inhibitory aptamer was generated through SELEX and in vivo screening.
  • Activity enhancement was attributed to altered complex formation stoichiometry, not higher binding affinity.
  • The modified aptamer demonstrated effective function as a transcriptional activator.

Conclusions:

  • Altering aptamer-protein complex stoichiometry is a viable strategy for enhancing aptamer activity.
  • This study presents a novel approach for aptamer engineering, diverging from affinity-based improvements.
  • Findings provide new insights for designing effective aptamer-based reagents and genetic components.