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Aspirin suppresses TNF-α-induced MMP-9 expression via NF-κB and MAPK signaling pathways in RAW264.7 cells

Ping Zhang1,2, Chao Wu3, Xiao-Hui Huang1

  • 1Department of Basic and Clinical Pharmacology, School of Pharmacy, Anhui Medical University, Hefei, Anhui 230032, P.R. China.

Experimental and Therapeutic Medicine
|December 30, 2017
PubMed

Insights

Aspirin reduces matrix metalloproteinase-9 (MMP-9) expression in macrophages by inhibiting NF-κB and MAPK pathways. This suggests aspirin

Area of Science:

  • Biochemistry
  • Pharmacology
  • Cell Biology

Background:

  • Matrix metalloproteinase-9 (MMP-9) expression is linked to atherosclerotic plaque rupture.
  • Aspirin is known for its anti-platelet effects in preventing atherosclerosis complications.

Purpose of the Study:

  • To investigate aspirin's effects on tumor necrosis factor-α (TNF-α)-induced MMP-9 expression in murine macrophages.
  • To elucidate the molecular mechanisms underlying aspirin's action on MMP-9.

Main Methods:

  • Western blot analysis to assess MMP-9 protein levels.
  • Quantitative polymerase chain reaction (qPCR) for MMP-9 mRNA expression.
  • Gelatin zymography assay to measure MMP-9 activity.
  • Inhibition of NF-κB and MAPK pathways to determine aspirin's mechanism.

Main Results:

  • Aspirin dose-dependently reduced MMP-9 protein, mRNA, and activity.
  • Aspirin suppressed NF-κB activation and MAPK phosphorylation (ERK1/2, p38, JNK).
  • Inhibiting MAPK pathways reduced MMP-9 expression, confirming aspirin's mechanism.

Conclusions:

  • Aspirin inhibits TNF-α-induced MMP-9 expression in macrophages.
  • Aspirin's mechanism involves suppressing NF-κB activation and the MAPK pathway.
  • Aspirin demonstrates therapeutic potential for preventing and treating atherosclerosis.
Keywords:
RAW264.7aspirinatherosclerosismatrix metalloproteinase-9mitogen-activated protein kinase

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