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Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice

Lu Liu1, Yu-Jie Lai2, Li-Ge Zhao1

  • 1Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, P.R. China.

Experimental and Therapeutic Medicine
|December 30, 2017
PubMed

Insights

Myc-interacting zinc-finger protein 1 (Miz1) expression is elevated in Alzheimer's disease (AD) mouse models. This protein is found in neurons and astrocytes, suggesting a role for Miz1 in AD pathogenesis.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pathology

Background:

  • Myc-interacting zinc-finger protein 1 (Miz1) is a transcription factor with known nuclear functions.
  • Miz1's role in cytoplasmic inflammation and its association with Alzheimer's disease (AD) pathogenesis are poorly understood.

Purpose of the Study:

  • To investigate the association between Alzheimer's disease and Miz1 expression in the brain.
  • To determine the cellular localization of Miz1 in AD mouse models.

Main Methods:

  • Utilized reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis to assess Miz1 expression levels.
  • Employed immunohistochemical and immunofluorescence staining to examine Miz1 distribution in wild-type and AD mouse brains.
  • Performed double immunofluorescence labeling to identify Miz1-expressing cell types, co-localizing with neuronal (MAP2) and astrocytic (GFAP) markers.

Main Results:

  • Miz1 expression was significantly upregulated in the cortex of AD mice compared to wild-type controls (P<0.05).
  • Miz1 protein was predominantly detected in neurons and astrocytes within the brain tissue.
  • Co-localization studies confirmed Miz1 presence in both neuronal and glial cells.

Conclusions:

  • Miz1 expression is elevated in the brains of AD mice.
  • Miz1 is localized in key brain cells, including neurons and astrocytes, relevant to AD pathology.
  • These findings suggest Miz1 plays a significant role in the pathogenesis of Alzheimer's disease.
Keywords:
APP/PS1 miceAlzheimer's diseaseMyc-interacting zinc-finger protein 1cognition

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