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Paeoniflorin exerts antitumor effects by inactivating S phase kinase-associated protein 2 in glioma cells

Jia Ouyang1, Hui Xu1, Ming Li1

  • 1Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.

Oncology Reports
|December 30, 2017
PubMed

Insights

Paeoniflorin (PF) inhibits glioma progression by downregulating S-phase kinase-associated protein 2 (Skp2). This natural compound shows promise as a therapeutic agent for glioma, targeting key oncogenic pathways.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Paeoniflorin (PF), derived from Paeoniae radix, exhibits antitumor properties against various cancers, including glioma.
  • The precise mechanism underlying PF's anti-glioma effects remains unclear.
  • S-phase kinase-associated protein 2 (Skp2) is recognized as an oncogene implicated in numerous cancer types.

Purpose of the Study:

  • To investigate if Skp2 mediates the anti-glioma activity of Paeoniflorin (PF).
  • To elucidate the molecular mechanisms by which PF exerts its effects on glioma cells.

Main Methods:

  • Assessing the impact of PF on glioma cell proliferation, migration, invasion, cell cycle progression (G2/M arrest), and apoptosis.
  • Analyzing Skp2 expression levels in PF-treated glioma cells.
  • Evaluating the effects of Skp2 overexpression and RNA interference on PF's anti-glioma actions.
  • Testing PF's efficacy in a U87 cell-derived xenograft mouse model.

Main Results:

  • Paeoniflorin (PF) significantly inhibited glioma cell proliferation, migration, and invasion.
  • PF treatment induced G2/M cell cycle arrest and promoted apoptosis in glioma cells.
  • PF downregulated Skp2 expression in glioma cells.
  • Overexpression of Skp2 abolished PF's anti-glioma effects, while Skp2 inhibition enhanced them.
  • PF treatment suppressed tumor growth in a xenograft mouse model.

Conclusions:

  • Paeoniflorin (PF) exerts its antitumor effects in glioma, at least partly, by inhibiting Skp2 expression.
  • PF demonstrates potential as a therapeutic agent for glioma treatment, targeting Skp2-mediated pathways.

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