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Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Nucleic Acid Structure01:25

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic Acids and Nucleotides01:20

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Visual Detection of Multiple Nucleic Acids in a Capillary Array.

Jianwei Chen1, Ning Shao1, Jiaying Hu2

  • 1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University; State Key Laboratory of Oncogenes and Related Genes; School of Biomedical Engineering, Shanghai Jiao Tong University.

Journal of Visualized Experiments : Jove
|December 30, 2017
PubMed
Summary
This summary is machine-generated.

A new Capillary Array-based Loop-mediated isothermal amplification for Multiplex visual detection (CALM) platform offers rapid, affordable, and visual detection of multiple nucleic acids. This technology enhances genetic analysis in fields like GMO detection and disease diagnostics.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Urgent need for rapid, affordable, and multiplex nucleic acid detection methods in diagnostics, microbial monitoring, GMO analysis, and forensics.
  • Previous development of the Capillary Array-based Loop-mediated isothermal amplification for Multiplex visual detection (CALM) platform.

Purpose of the Study:

  • To describe improved fabrication and performance processes for the CALM platform.
  • To demonstrate the platform's utility for multiplex visual detection of nucleic acids.
  • To showcase high-throughput nucleic acid analysis capabilities.

Main Methods:

  • Utilized a small, ready-to-use cassette assembled by a patterned capillary array.
  • Pre-treated capillaries with hydrophobic and hydrophilic patterns, immobilizing loop-mediated isothermal amplification (LAMP) primer sets.
  • Single pipetting step to load reaction mixture, with capillary forces isolating reactants in parallel within each capillary for visual readout via UV illumination.

Main Results:

  • Successfully demonstrated the monitoring of 8 frequently appearing elements and genes in genetically modified organism (GMO) samples.
  • Achieved high specificity and sensitivity in GMO detection using the improved CALM platform.
  • Visual readout by UV flashlight confirmed the presence of target nucleic acids.

Conclusions:

  • The improved CALM platform facilitates efficient detection of multiple nucleic acids.
  • The system is user-friendly, resource-affordable, and suitable for rapid analysis.
  • The technology holds significant potential for widespread application in high-throughput nucleic acid analysis across various scientific fields.