Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What are Viruses?00:50

What are Viruses?

128.5K
Overview
128.5K
Drug Discovery: Overview01:26

Drug Discovery: Overview

12.0K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
12.0K
Appendicitis-II: Diagnostic Studies and Management01:29

Appendicitis-II: Diagnostic Studies and Management

604
Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
604
Myasthenia Gravis: Diagnostic Tests01:15

Myasthenia Gravis: Diagnostic Tests

2.7K
Myasthenia gravis is an autoimmune condition affecting neuromuscular transmission, causing generalized weakness in skeletal muscles. Initial diagnoses rely on patients' signs, symptoms, and medical history. The challenge lies in distinguishing myasthenia from other muscular dystrophies. An important diagnostic feature is the significant improvement of symptoms after administering anticholinesterase inhibitors.
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
2.7K
Asthma-IV: Diagnostic and Management01:30

Asthma-IV: Diagnostic and Management

3.2K
The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
3.2K
Specific Heat01:16

Specific Heat

67.9K
The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
67.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of Powassan Virus Seropositivity Among People with History of Lyme Disease and Non-Lyme Community Controls in the Northeastern United States.

Vector borne and zoonotic diseases (Larchmont, N.Y.)·2024
Same author

Development and Evaluation/Verification of a Fully Automated Test Platform for the Rapid Detection of <i>Cyclospora cayetanensis</i> in Produce Matrices.

Microorganisms·2023
Same author

SARS-CoV-2 mRNA vaccination induces an antigen-specific T cell response correlating with plasma interferon-gamma in B cell depleted patients.

Journal of neuroimmunology·2023
Same author

The need for fast and accurate detection of dermatomycosis.

Medical mycology·2023
Same author

Study of efficacy and longevity of immune response to third and fourth doses of COVID-19 vaccines in patients with cancer: A single arm clinical trial.

eLife·2023
Same author

Periodontal Inflamed Surface Area (PISA) associates with composites of salivary cytokines.

PloS one·2023

Related Experiment Video

Updated: Feb 16, 2026

Zika Virus Specific Diagnostic Epitope Discovery
11:37

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

8.3K

Zika Virus Specific Diagnostic Epitope Discovery.

Maite Sabalza1, Cheryl A Barber2, William R Abrams2

  • 1Department of Basic Sciences and Craniofacial Development, New York University College of Dentistry; ms10129@nyu.edu.

Journal of Visualized Experiments : Jove
|December 30, 2017
PubMed
Summary

This study presents a protocol for discovering Zika virus (ZIKV) diagnostic peptides using high-density peptide microarrays. The method identifies specific peptides that bind to Zika virus antibodies in human serum for diagnostic development.

More Related Videos

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
10:01

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Published on: October 17, 2018

8.5K
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
07:35

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Published on: June 7, 2017

10.2K

Related Experiment Videos

Last Updated: Feb 16, 2026

Zika Virus Specific Diagnostic Epitope Discovery
11:37

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

8.3K
Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
10:01

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Published on: October 17, 2018

8.5K
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
07:35

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Published on: June 7, 2017

10.2K

Area of Science:

  • Biotechnology
  • Virology
  • Immunology

Background:

  • High-density peptide microarrays enable high-throughput screening of thousands of peptides.
  • These arrays are valuable tools for drug discovery, target identification, and diagnostics development.

Purpose of the Study:

  • To present a protocol for identifying specific Zika virus (ZIKV) diagnostic peptides.
  • To demonstrate the utility of high-density peptide microarrays in ZIKV research.

Main Methods:

  • A high-density peptide microarray was designed with 3,423 unique 15-amino acid peptides from the ZIKV proteome.
  • A human serum sample confirmed for ZIKV infection was incubated with the microarray.
  • Secondary antibodies targeting Immunoglobulin M (IgM) and Immunoglobulin G (IgG) were used for peptide detection.

Main Results:

  • The study successfully identified specific peptides that bind to ZIKV-specific IgM and IgG antibodies in human serum.
  • These identified peptides are candidates for further validation in diagnostic assays.

Conclusions:

  • High-density peptide microarrays provide an effective strategy for discovering diagnostic peptides for viral infections like ZIKV.
  • The described protocol facilitates the design, processing, and analysis of peptide microarrays for diagnostic applications.