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Updated: Feb 16, 2026
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Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
Published on: November 17, 2017
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Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
Sander Bekeschus1, Anke Schmidt2, Felix Niessner2
1ZIK plasmatis, Leibniz-Institute for Plasma Science and Technology; sander.bekeschus@inp-greifswald.de.
Journal of Visualized Experiments : Jove
|December 30, 2017
Summary
This study details essential equipment and workflows for plasma biomedicine research. It enables reproducible analysis of plasma-treated cells, advancing medical applications like wound healing and cancer therapy.
Area of Science:
- Plasma medicine
- Biomedical engineering
- Cell biology
Background:
- Cold atmospheric plasmas generate reactive species influencing biological processes.
- Plasma treatment shows promise for chronic wound healing and cancer therapy.
- Plasma medical research demands interdisciplinary expertise.
Purpose of the Study:
- To describe essential equipment and workflows for plasma biomedicine research.
- To enable reproducible characterization of plasma-treated cells.
- To establish protocols for analyzing cellular responses to plasma treatment.
Main Methods:
- Operation of an atmospheric pressure argon plasma jet with emission spectra monitoring.
- High-precision XYZ-table and software for reproducible plasma application to 96-well plates.
- Sequential assays on melanoma cells for metabolic activity, cell area, and calreticulin expression.
Main Results:
- Demonstration of reproducible plasma treatment delivery with millisecond and micrometer precision.
- Methodology for downstream analysis of redox-active molecules in liquid samples.
- Efficient multi-assay analysis of plasma-treated melanoma cells on a single plate.
Conclusions:
- The study provides essential protocols for plasma medical research.
- The described methods allow for content-rich biological data retrieval from plasma-treated cells.
- This work facilitates advancements in plasma-based therapeutic applications.
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