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Related Concept Videos

Aging01:26

Aging

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Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
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Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
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Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
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Changes in the Appendicular Skeleton with Age01:09

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The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
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Bonding and Strength of Aggregate01:12

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The bond between aggregate particles and the cement matrix is significantly influenced by the shape and surface texture of the aggregates. High-strength concretes benefit from a rougher texture, which leads to stronger bonding due to greater adhesion. Angular aggregates with larger surface areas also enhance this bond. The bonding quality, however, is complex to assess as no universally accepted test exists. Good bonding is indicated when a crushed concrete specimen shows some aggregate...
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Specific Gravity of Aggregate01:19

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Aggregates typically contain pores, which can be either permeable or impermeable. Considering the pores in the aggregates, the specific gravity of aggregates is defined in three different forms, namely, bulk or gross specific gravity, apparent specific gravity, and absolute specific gravity.
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Related Experiment Video

Updated: Feb 16, 2026

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans.

Nicole Groh1, Ivan Gallotta2, Marie C Lechler1

  • 1Protein Aggregation and Aging, German Center for Neurodegenerative Diseases (DZNE); Graduate School of Cellular and Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE).

Journal of Visualized Experiments : Jove
|December 30, 2017
PubMed
Summary
This summary is machine-generated.

Protein aggregation is a normal part of aging, not just disease. This study presents methods to study age-dependent protein aggregation in worms, aiding research into neurodegenerative disorders like Alzheimer's disease and Parkinson's disease.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Neurodegenerative disorders like Alzheimer's disease (AD) and Parkinson's disease (PD) are increasing.
  • These diseases involve the aggregation of proteins into fibrils in the brain.
  • The exact reasons for protein aggregation during aging are not fully understood.

Purpose of the Study:

  • To develop and present methodologies for studying inherent protein aggregation in aging Caenorhabditis elegans.
  • To investigate the molecular and cellular mechanisms regulating age-dependent protein aggregation.
  • To provide tools for understanding why proteins aggregate with age and how to maintain their function.

Main Methods:

  • Culturing age-synchronized Caenorhabditis elegans to obtain aged animals.
  • Biochemical isolation of highly insoluble protein aggregates.
  • Targeted genetic knockdown combined with quantitative mass spectrometry or antibody-based analysis.
  • In vivo validation using transgenic animals expressing fluorescent-tagged aggregation-prone proteins.

Main Results:

  • Established protocols for studying age-dependent protein aggregation in C. elegans.
  • Demonstrated methods to dissect the role of specific genes in protein aggregation.
  • Validated findings through complementary biochemical and in vivo approaches.

Conclusions:

  • Protein aggregation is a natural process during aging.
  • The presented methodologies enable the study of inherent protein aggregation.
  • This research can elucidate mechanisms of protein aggregation and inform strategies for maintaining protein function in aging and disease.