Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pancreas01:19

Pancreas

2.5K
The pancreas, an essential organ in the human body, is a pinkish-gray elongated structure located posterior to the stomach. It extends laterally from the duodenum towards the spleen and is firmly bound to the posterior wall of the abdominal cavity. The organ's surface has a lumpy, lobular texture that gives it a unique appearance.
The broad head of the pancreas lies within the loop formed by the duodenum, while its slender body reaches towards the spleen. The tail of the pancreas is short...
2.5K
Cells and Secretions of the Pancreas01:16

Cells and Secretions of the Pancreas

5.4K
The pancreas, a vital organ within the abdominal cavity, plays dual roles in the digestive and endocrine systems, collaborating with exocrine and endocrine cells to maintain optimal digestion and blood sugar levels.
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
5.4K
Modified Boxplots00:57

Modified Boxplots

11.3K
A standard box and whisker plot informs us about the spread of the data in a given sample. One can identify the minimum value, maximum value, first quartile value, second quartile or median value, and third quartile.
However, the box plot does not tell the reader about outliers - values that lie far from the center of the data. We can modify the standard box and whisker plot to identify the outliers and visualize the actual spread of the data in a sample.
Initially, we calculate the adjusted...
11.3K
Tissue Transplantation01:24

Tissue Transplantation

1.1K
Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
1.1K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.6K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.6K
Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

514
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
514

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Renal Function Decline After Vascularized Composite Allotransplantation.

Transplantation direct·2026
Same author

Implementation of a controlled DCD program in Western Austria - key considerations and insights.

Transplant international : official journal of the European Society for Organ Transplantation·2026
Same author

The integration of psychosocial care, ethical governance, and patient-centered research in current and future approaches in the field of vascularized composite allotransplantation.

Frontiers in transplantation·2026
Same author

Correction: Effect of normothermic machine perfusion on glycocalyx shedding during liver transplantation - a prospective pilot study.

Transplant international : official journal of the European Society for Organ Transplantation·2026
Same author

International multisociety Delphi consensus for liver tumour thermal ablation: margin assessment.

The Lancet. Oncology·2026
Same author

International multisociety Delphi consensus for liver tumour thermal ablation: procedural and practice standards.

The Lancet. Oncology·2026

Related Experiment Video

Updated: Feb 16, 2026

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique
13:19

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Published on: December 16, 2017

11.6K

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique.

Benno Cardini1, Rupert Oberhuber1, Sven R Hein1

  • 1Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck.

Journal of Visualized Experiments : Jove
|December 30, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel non-suture cuff technique for mouse pancreas transplantation, achieving over 90% success. This method minimizes early graft damage from ischemia reperfusion injury, advancing transplantation research.

More Related Videos

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
10:32

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Published on: October 12, 2014

14.9K
A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
06:45

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Published on: February 7, 2022

4.2K

Related Experiment Videos

Last Updated: Feb 16, 2026

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique
13:19

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Published on: December 16, 2017

11.6K
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
10:32

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Published on: October 12, 2014

14.9K
A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
06:45

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Published on: February 7, 2022

4.2K

Area of Science:

  • Transplantation research
  • Surgical innovation
  • Murine models

Background:

  • Mouse models are crucial for transplantation research due to genetic diversity and reagent availability.
  • Early graft damage from ischemia reperfusion injury is a significant challenge in pancreas transplantation.
  • Existing suture techniques have limitations in mouse pancreas transplantation models.

Purpose of the Study:

  • To develop and describe a novel, highly successful heterotopic mouse pancreas transplantation model.
  • To analyze mechanisms of early graft damage associated with ischemia reperfusion injury.
  • To present a non-suture cuff technique for improved graft revascularization.

Main Methods:

  • A heterotopic pancreas transplantation model in mice using a non-suture cuff technique.
  • Arterial anastomosis: donor aorta over everted recipient common carotid artery.
  • Venous anastomosis: donor portal vein over everted recipient external jugular vein.
  • Graft revascularization involves vessel eversion, cuff placement, and ligature fixation.

Main Results:

  • Over 300 successful mouse pancreas transplantations performed with a >90% success rate.
  • The non-suture cuff technique demonstrated high patency rates and reduced thrombogenic lesions.
  • The technique ensures endothelial layer continuity, crucial for graft survival.

Conclusions:

  • The described non-suture cuff technique offers a highly reproducible and successful method for mouse pancreas transplantation.
  • This model facilitates the study of ischemia reperfusion injury and early graft damage mechanisms.
  • The detailed procedure enables microsurgical researchers to implement this advanced transplantation technique.