Related Experiment Video
Updated: Feb 16, 2026

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
Published on: February 14, 2022
Optimization for imaging through scattering media for confocal microscopes with divided elliptical apertures
Hengjie Tang1, Chenxue Wu1, Wei Gong2
1State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.
Abstract:
We develop a confocal system equipped with optimal elliptical apertures to improve axial point spread function and signal-to-background ratio (SBR) for different detector sizes. By adjusting the parameters of the elliptical apertures, the axial half width at half-maximum can be reduced to 4.986 (described in optical coordinates) and SBR can be improved to 0.176. We evaluate this system with the 1951 USAF resolution test chart and the primary cultured neuron from SD rat stained by Map-2, and observe better imaging performance, which indicates the potential applications in biological science.
Related Concept Videos
Voltage Dividers
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...
Current Dividers
Scatter Plot
Optimal Foraging
Microbial Growth Media
Optimization Problems

