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Updated: Feb 16, 2026

Use of In Vivo Assembly for High-efficiency Plasmid Construction
Published on: February 7, 2025
Capture efficiency of portable high-efficiency air filtration devices used during building construction activities
Derek A Newcomer1, Peter LaPuma2, Robert Brandys3
1a National Institutes of Health , Bethesda , Maryland.
Abstract:
The portable high-efficiency air filtration (PHEAF) device is used to control particulate matter (PM) generated from construction-type activities occurring within the built environment. Examples of activities where PHEAF devices are mobilized include building renovation, asbestos abatement, remediation of microbial contamination, and lead-based paint projects. Designed for use on short-term, temporary projects the PHEAF device captures airborne PM using a high-efficiency particulate air (HEPA) filter. This study sought to evaluate the capture efficiency of the PHEAF device in a field setting. An aerosol generator and photometer were used to measure particle penetration through 85 PHEAF devices. Average overall capture efficiency ranged from 41.78% to ≥99.97% with more than 88% of the tests failing to achieve 99.97% capture efficiency. Approximately 73% of the PHEAF device sample population failed to demonstrate HEPA performance criteria during any test round. A higher occurrence of PM concentrations measured around the perimeter of the filter suggested the presence of bypass leakage. While PHEAF devices were effective in capturing a significant quantity of aerosol test agent, these findings suggest that routine testing of the PHEAF device should be conducted to validate performance.
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