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Updated: Feb 16, 2026

A Blood-Free Diet to Rear Anopheline Mosquitoes
Published on: January 31, 2020
A highly stable blood meal alternative for rearing Aedes and Anopheles mosquitoes
Ted Baughman1, Chelsea Peterson1, Corrie Ortega1
1Intellectual Ventures Laboratory and Global Good, Bellevue, Washington, United States of America.
Abstract:
We investigated alternatives to whole blood for blood feeding of mosquitoes with a focus on improved stability and compatibility with mass rearing programs. In contrast to whole blood, an artificial blood diet of ATP-supplemented plasma was effective in maintaining mosquito populations and was compatible with storage for extended periods refrigerated, frozen, and as a lyophilized powder. The plasma ATP diet supported rearing of both Anopheles and Aedes mosquitoes. It was also effective in rearing Wolbachia-infected Aedes mosquitoes, suggesting compatibility with vector control efforts.
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