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miR-365 functions as a tumor suppressor by directly targeting CYR61 in osteosarcoma

Yawei Xu1, Haijiao Chu1, Yan Zhou1

  • 1College of Bioengineering, Jilin Agricultural Science and Technology University, Jilin City 132101, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|December 30, 2017
PubMed

Insights

MicroRNAs (miRNAs) like miR-365 are downregulated in osteosarcoma (OS), inhibiting tumor growth. Targeting CYR61, miR-365 acts as a tumor suppressor in OS by reducing proliferation and metastasis.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression implicated in various cancers.
  • Aberrant miRNA expression is frequently observed in osteosarcoma (OS), suggesting their role in tumorigenesis.

Purpose of the Study:

  • To investigate the functional role of miR-365 in osteosarcoma (OS).
  • To elucidate the underlying molecular mechanism of miR-365 action in OS cells.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess miR-365 expression in OS tissues and cell lines.
  • Cell proliferation, migration, and invasion assays in OS cells with miR-365 overexpression.
  • Western blotting and luciferase reporter assays to identify and validate miR-365 targets.

Main Results:

  • miR-365 expression was significantly downregulated in OS tissues and cell lines, correlating with advanced clinical stage, metastasis, and poor survival.
  • Overexpression of miR-365 suppressed proliferation, migration, and invasion in Saos-2 and MG-63 OS cells.
  • Cysteine-rich angiogenic inducer 61 (CYR61) was identified as a direct target of miR-365, with its expression inversely correlated with miR-365 levels in OS. CYR61 overexpression counteracted miR-365's tumor-suppressive effects.

Conclusions:

  • miR-365 functions as a tumor suppressor in osteosarcoma.
  • miR-365 exerts its tumor-suppressive functions, at least partly, by targeting and downregulating CYR61 expression in OS cells.
Keywords:
CYR61InvasionOsteosarcomaProliferationmiR-365

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