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Published on: July 30, 2011
Microarray analysis of lncRNA and mRNA expression profiles in mice with allergic rhinitis
Yue Ma1, Le Shi1, Chunquan Zheng1
1Department of Otolaryngology-Head and Neck Surgery, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai Key Clinical Disciplines of Otorhinolaryngology, PR China.
Objectives:
We aimed to identify the effect of lncRNAs in CD4+ T cells on Allergic rhinitis (AR).
Methods:
The present study conducted a microarray to identify the expression profiles of lncRNA and mRNA in CD4+ T cells in both AR murine models and normal controls. And qRT-PCR was used to confirm the results. GO and KEGG enrichment analysis were used to show all related pathways and a co-expression network was conducted to find lncRNAs which have high correlation with these pathways.
Results:
The results showed that the two groups contained a total of 158 deregulated lncRNAs, of which 110 were upregulated and 48 were downregulated. And positive regulation of calcium ion transport, B cell activation, chemokine-signaling pathways and calcium-signaling pathways may be involved in the development of T cells in AR pathology. Finally, we can find the differentially expressed mRNA in the pathways related to T cell differentiation correlated with many deregulated lncRNAs.
Conclusions:
The present study was the first to show the differential expression profiles of lncRNAs in the CD4+ T cells of an AR murine model, which may provide significant insights into AR pathogenesis and offer new treatment targets to alleviate it.
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