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Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 714 adults from Colombo, Sri Lanka

Alba Grifoni1, Daniela Weiskopf1, Cecilia S Lindestam Arlehamn1

  • 1Division of Vaccine Discovery, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA.

Human Immunology
|January 1, 2018
PubMed

Insights

This study characterized human leukocyte antigen (HLA) allele frequencies in Sri Lankan blood donors. High frequencies of specific HLA class I and II alleles were identified, crucial for understanding T cell immunity to pathogens like Dengue virus.

Area of Science:

  • Immunogenetics
  • Population Genetics
  • Molecular Biology

Background:

  • The human leukocyte antigen (HLA) complex plays a critical role in immune responses.
  • Understanding HLA allele frequencies is essential for studying T cell immunity against various pathogens.
  • Previous data on HLA diversity in Sri Lanka is limited.

Purpose of the Study:

  • To determine the allele frequencies of HLA-A, -B, -C, -DPB1, -DQA1, -DQB1, and -DRB1 loci in a Sri Lankan population.
  • To provide foundational data for immunological studies, particularly concerning T cell responses to infectious diseases such as Dengue virus.

Main Methods:

  • DNA sequencing was employed for high-resolution typing of HLA loci.
  • 714 healthy adult blood bank donors from Colombo, Sri Lanka, were included in the study.
  • Allele frequencies were calculated and compared against Hardy-Weinberg proportions.

Main Results:

  • No significant deviations from Hardy-Weinberg equilibrium were observed across all tested HLA loci.
  • Several HLA alleles exhibited high frequencies (>30%), including DPB1*04:01, DPB1*02:01, DQB1*06:01, DRB1*07:01 (class II), and A*33:03, A*24:02 (class I).
  • Genotype data will be publicly accessible via the Allele Frequencies Net Database.

Conclusions:

  • This study establishes a comprehensive HLA allele frequency profile for the Sri Lankan population.
  • The identified high-frequency alleles provide valuable insights for future research into HLA-associated immune responses in this region.
  • The data supports investigations into pathogen-specific T cell immunity, including responses to Dengue virus.

Keywords:
Colombo, Sri LankaHLA allelesHLA typingMoorsSinhaleseTamil

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