Related Experiment Video
Updated: Feb 16, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Synthesis of 2-docosahexaenoylglycerol by enzymatic ethanolysis
Yu Zhang1, Xiaosan Wang1, Shuo Zou1
1Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
Abstract:
Synthesis of 2-docosahexaenoylglycerol with high nutritional value was conducted by enzymatic ethanolysis of algal oil. The effects of lipase type, substrate molar ratio of algal oil to ethanol, reaction time, reaction temperature and lipase load on the content of 2-monoacylglycerols (2-MAGs) in the crude product were investigated. Under the optimal conditions, 27-31% 2-MAGs were obtained in the ethanolysis reaction. Lipozyme 435 exhibited 1,3-specific selectivity and maintained stable operational stability after 7 successive reuse cycles. The enzymatic ethanolysis catalyzed by Lipozyme 435 could both synthesize 2-MAGs and concentrate DHA. Further purification of 2-MAGs was performed with solvent extraction by 85% ethanol aqueous solution and hexane, obtaining 95% 2-MAGs in a yield of 67%. The contents of DHA in 2-MAGs product and 2-MAGs fraction were 74.76% and 75.66%, respectively, which were about 26% higher than that in the algal oil. Therefore, this method is efficient and environmental-friendly for synthesis of 2-docosahexaenoylgylcerol.
Related Concept Videos
Dehydration Synthesis
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
Synthesis and Decomposition Reactions
Lagging Strand Synthesis
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
Transfer RNA Synthesis
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Amplifying Signals via Enzymatic Cascade
meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H

