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Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Establishment and characterization of induced pluripotent stem cells from placental mesenchymal stromal cells

Shagufta Parveen1

  • 1School of Regenerative Medicine, Manipal University, GKVK Post, Bellary Road, Bengaluru 560065, Karnataka, India.

Stem Cell Research
|January 2, 2018
PubMed

Human term placenta is a bulky organ which harbours abundant mesenchymal stromal cells. This study reports isolation and characterization of placental mesenchymal stromal cells (PMSCs) followed by reprogramming of PMSCs to induced pluripotent stem cells (iPSCs). The placental human iPSC (PhiPSC) line is pluripotent with high telomerase activity, genetically identical to parental PMSCs, transgene free, karyotypically normal and differentiates into ectoderm, mesoderm and endoderm both in vitro and in vivo. Thus PMSCs serve as a unique cell type for human perinatal iPSC derivation from extra embryonic tissue.

Keywords:
Human term placentaKaryotypically normalPlacental human induced pluripotent stem cellsPlacental mesenchymal stromal cellsTeratomaTransgene free

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