Comparison of international methods for the determination of total starch in raw sugars: Part II
Marsha Cole1, Gillian Eggleston2
1USDA-ARS Southern Regional Research Center, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, USA; Oak Ridge Institute for Science Education Research Participation Program at USDA-ARS, USA.
Abstract:
Industrial starch methods in the sugar industry are affected by sugarcane- and processing-derived colourants, and it was presumed that these methods are mathematically equatable. Using the USDA Starch Research method as a reference and factory raw sugars, the impact of colourants on the accuracy, precision, limits of detection/quantification, and mathematical equatability of the starch methods were investigated. Approximately 26-55% of raw sugar colour contributed to starch-I3- absorbance. The exclusion of a colour blank negatively affected method accuracy and the addition of a colour blank confirmed that these methods measured mostly colour instead of starch. Inefficient starch solubilization and the inability to standardize sugar colourants explained why starch results from these methods could not be mathematically equated to the USDA Starch Research method, or among different methods. An industrial starch method that efficiently solubilizes starch and includes a colour blank is urgently needed.
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Phloem and Sugar Transport
Internal Energy
Internal Receptors
Renal Drug Clearance: Comparison Between Renal Excretion Methods
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Sugars as Energy Storage Molecules


