Draft genome sequence of CTX-M-type β-lactamase-producing Klebsiella quasipneumoniae subsp. similipneumoniae isolated

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Chien-Feng Li1, Hui-Ling Tang2, Chien-Shun Chiou3

  • 1Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.

Journal of Global Antimicrobial Resistance
|January 3, 2018
PubMed
Abstract

Insights

We sequenced the genome of Klebsiella quasipneumoniae subsp. similipneumoniae strain CHKP0062 from a turtle, finding it carries important antibiotic resistance genes like CTX-M-type beta-lactamase. This provides insights into antimicrobial resistance gene spread.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Klebsiella species are significant pathogens in human and animal infections.
  • Understanding the genetic basis of antimicrobial resistance in Klebsiella is crucial for public health.

Purpose of the Study:

  • To report the draft genome sequence of a Klebsiella quasipneumoniae subsp. similipneumoniae strain (CHKP0062) isolated from a Yellow-margined Box turtle.
  • To investigate the antimicrobial resistance and virulence genes present in this strain.

Main Methods:

  • Whole-genome sequencing using an Illumina-Solexa platform.
  • De novo assembly using Velvet and annotation via the NCBI Prokaryotic Genome Annotation Pipeline (PGAP).
  • Analysis of the resistome and virulome, including plasmid assembly.

Main Results:

  • The 5.7 Mb genome contains 5423 protein-coding sequences, 87 tRNAs, 24 rRNAs, and 12 ncRNAs.
  • Strain CHKP0062 belongs to sequence type ST2131 and K-loci type KL67.
  • No virulence genes were detected, but multiple antimicrobial resistance genes were identified, including blaTEM-1b and blaCTX-M-3 on plasmids homologous to known multidrug resistance plasmids.

Conclusions:

  • The genome sequence provides a valuable resource for comparative genomics.
  • It aids in understanding the dissemination of clinically important antibiotic resistance genes in Klebsiella spp. between animal and human populations.
Keywords:
Box turtleCTX-MKlebsiella quasipneumoniaeTEM

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