Related Experiment Video

Updated: Feb 16, 2026

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
09:45

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Published on: August 25, 2014

19.2K

[Effects of propofol on myelin basic protein expression in oligodendrocytes of SD rats at different developmental

Xiao-Qin Zhu1, Chun-Shui Lin, Pei-Pei Guo

  • 1Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|January 3, 2018
PubMed

Insights

Propofol reduces myelin basic protein (MBP) expression in young rats, particularly at 7 and 14 days old. This effect was observed at both mRNA and protein levels, with increased caspase-3 expression in younger animals.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Pharmacology

Background:

  • Oligodendrocytes are crucial for myelin sheath formation in the central nervous system.
  • Myelin basic protein (MBP) is a key component of the myelin sheath.
  • Propofol is a widely used anesthetic agent with potential neurotoxic effects.

Purpose of the Study:

  • To investigate the impact of propofol on myelin basic protein (MBP) expression in Sprague-Dawley (SD) rats.
  • To assess these effects across different developmental stages (3, 7, 14, and 21 days old).

Main Methods:

  • SD rats of varying ages (3, 7, 14, 21 days) were administered propofol or a control emulsion.
  • Quantitative real-time PCR (qRT-PCR) was used to measure mRNA levels of MBP and caspase-3.
  • Western blotting and immunohistochemistry analyzed MBP protein expression.

Main Results:

  • Propofol significantly downregulated MBP mRNA and upregulated caspase-3 mRNA in 3, 7, and 14-day-old rats.
  • MBP protein levels were significantly decreased in 7 and 14-day-old rats following propofol administration.
  • No significant differences in caspase-3 mRNA or MBP protein were observed in 21-day-old rats.

Conclusions:

  • Propofol exposure negatively affects MBP expression in developing SD rats.
  • The most pronounced effects were observed in rats at 7 and 14 days of age.
  • These findings suggest propofol may interfere with oligodendrocyte development and myelination during critical early life stages.
Abstract

More Related Videos

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues18:17

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

Published on: August 21, 2011

37.2K
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays10:00

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Published on: January 12, 2015

15.1K

Related Concept Videos

Energy Basics02:27

Energy Basics

Chemical reactions, such as those that occur when you light a match, involve changes in energy as well as matter.
47.9K
Introduction to Developmental Psychology01:27

Introduction to Developmental Psychology

Developmental psychology explores the changes and continuities in human abilities throughout life, encompassing physical, cognitive, linguistic, and social dimensions. Human development is not restricted to growth, but includes aspects of decline, particularly in physical abilities as individuals age. Developmental psychologists seek to understand how people change as they age and how their mental and social skills evolve.Developmental MilestonesA key concept in developmental psychology is...
1.7K
Three Developmental Domains01:29

Three Developmental Domains

Human development is typically examined across three main domains: physical, cognitive, and socio-emotional. These domains represent the significant areas of change and continuity throughout the lifespan, from infancy to late adulthood.
Physical Development
Physical processes, also known as maturation, encompass the biological changes that occur across an individual's life. These changes begin with genetic inheritance and continue through various stages, including growth in height and weight,...
1.2K
Nervous Tissue: Myelin01:25

Nervous Tissue: Myelin

The myelin sheath is a multilayered lipid and protein covering that insulates the axon of a neuron, enhancing the speed of nerve impulse conduction. Axons without this sheath are referred to as unmyelinated. Two types of neuroglia, Schwann cells in the peripheral nervous system (PNS) and oligodendrocytes in the central nervous system (CNS) are responsible for producing myelin sheaths.
Schwann cells begin to form myelin sheaths around axons during fetal development. They wrap around a small...
6.0K
Basicity of Aliphatic Amines01:21

Basicity of Aliphatic Amines

Amines can behave as Brønsted–Lowry bases by accepting a proton from the acid to form corresponding conjugate acids. Due to a lone pair of nonbonding electrons, aliphatic amines can also act as Lewis bases by forming a covalent bond with an electrophile.
To measure the basicity of amines, two conventions are generally used. The first defines Kb as the basicity constant for the deprotonation reaction of water by the amine, as presented in Figure 1. Conventionally, lower Kb indicates higher...
6.9K
Basic Operations on Signals01:22

Basic Operations on Signals

Basic signal operations include time reversal, time scaling, time shifting, and amplitude transformations. These operations are fundamental in signal processing and analysis.
Time Reversal mirrors a continuous-time signal about the vertical axis at t=0. This is achieved by substituting t with −t. For example, if a signal x(t) is considered, the time-reversed signal is x(−t). This operation can be graphically represented, showing the mirrored signal.
1.1K