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Updated: Feb 16, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.7K

Lem2 is retained at the nuclear envelope through its interaction with Bqt4 in fission yeast

Yasuhiro Hirano1, Yasuha Kinugasa1, Haruhiko Asakawa1

  • 1Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|January 3, 2018
PubMed

Insights

Inner nuclear membrane protein Lem2 interacts with Bqt4 for nuclear envelope retention. Loss of Bqt4 causes Lem2 to accumulate at the spindle pole body, revealing distinct N-terminal and C-terminal Lem2 functions.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Inner nuclear membrane (INM) proteins regulate nuclear organization and function via chromatin interactions.
  • Redundant functions of INM proteins in metazoans complicate functional studies.
  • Fission yeast Schizosaccharomyces pombe offers a simpler model with fewer INM proteins and available genetic tools.

Purpose of the Study:

  • To investigate the functional relationship between INM proteins Lem2 and Bqt4 in S. pombe.
  • To elucidate the mechanism of Lem2 retention at the nuclear envelope.
  • To understand the distinct roles of Lem2 domains in its interaction with Bqt4.

Main Methods:

  • Genetic analysis of double mutants (lem2 and bqt4) in S. pombe.
  • Localization studies of Lem2 protein in wild-type and mutant backgrounds.
  • Domain-specific analysis of Lem2 function.

Main Results:

  • Lem2 is retained at the nuclear envelope through interaction with Bqt4.
  • Loss of Bqt4 causes Lem2 to accumulate at the spindle pole body (SPB).
  • The N-terminal region of Lem2 competitively binds to Bqt4 and the SPB.
  • The C-terminal region of Lem2 suppresses the synthetic lethality of the lem2 bqt4 double mutant.

Conclusions:

  • Lem2 localization to the nuclear envelope depends on Bqt4.
  • Lem2 possesses independent N-terminal and C-terminal functional domains.
  • These domains mediate distinct interactions critical for nuclear organization and cellular viability.
Keywords:
Schizosaccharomyces pombeBqt4DNA bindingLem2Man1nuclear membranespindle pole body

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