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Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
Published on: November 1, 2011
Med1 deficiency alters the somatic hypermutation spectrum in murine B cells
Léa Gaudot1,2,3,4, Anne-Sophie Thomas-Claudepierre1,2,3,4, Isabelle Robert1,2,3,4
1Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Illkirch, France.
Abstract:
The Mediator complex is known to orchestrate transcription. Here we show that B cell conditional deficient mice for the Med1 subunit display robust somatic hypermutation. Nevertheless, the mutation frequency at A residues is decreased and the expected A/T ratio is abolished, implicating Mediator in the second phase of somatic hypermutation.
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