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High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
Published on: March 12, 2012
The cold tolerance of the northern root-knot nematode, Meloidogyne hapla
Xiaojing Wu1, Xiaofeng Zhu1, Yuanyuan Wang2
1College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, China.
Abstract:
The northern root-knot nematode, Meloidogyne hapla, is one of the most important nematode pathogens occurring in cold regions. It is a sedentary, biotrophic parasites of plants and overwinter in the soil or in diseased roots. This study showed that the cold tolerance for the second-stage juveniles (J2) of M. hapla was moderate with the 50% survival temperature (S50) of -2.22°C and the fatal temperature was -6°C when cooling at 0.5°C min-1. Cryoprotective dehydration significantly enhance cold tolerance of M. hapla J2 with the lowest S50 of -3.28°C after held being at -1°C for 6 h. Moreover, cold shock and cold acclimation had significant effects on the freezing survival of M. hapla J2. The lethal temperature of eggs was -18°C. Therefore, the cold tolerance of M. hapla is sufficiently favorable to withstand winters in cold temperature environments.
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