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10:11
Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
Published on: September 5, 2012
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Ethanol toxicity differs depending on the time of day
Luisa M Vera1,2, Carolina Bello1, Juan F Paredes1
1Department of Physiology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia, Spain.
Plos One
|January 3, 2018
Summary
Ethanol toxicity and behavior in zebrafish show significant daily rhythms. Morning exposure to ethanol resulted in higher mortality and altered swimming activity compared to night exposure, highlighting time-dependent effects.
Area of Science:
- Chronobiology
- Toxicology
- Neuroscience
Background:
- Ethanol is a widely abused substance with known toxic and psychoactive effects.
- However, the time-dependent impact of ethanol exposure remains under-investigated.
Purpose of the Study:
- To investigate daily rhythms in ethanol toxicity and behavioral responses in zebrafish.
- To analyze the temporal expression patterns of key ethanol detoxification genes.
Main Methods:
- Zebrafish larvae and adults were exposed to ethanol at different times of day.
- Mortality rates, swimming activity, and gene expression (adh8a, adh5, aldh2.1, aldh2.2) were assessed.
Main Results:
- Significant daily rhythm in ethanol toxicity observed, with higher mortality in the morning (82%) versus night (6%).
- Ethanol exposure altered swimming behavior, decreasing it in the morning/day and increasing it at night.
- Ethanol detoxification genes showed circadian regulation in constant darkness, but not in light-dark cycles.
Conclusions:
- Time of day significantly influences ethanol's toxic and behavioral effects in zebrafish.
- Chronotoxicity and chronobehavioral effects of ethanol are evident, necessitating consideration of exposure timing in research and clinical settings.
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