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Updated: Feb 16, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Published on: March 6, 2018
Correction to: Deformability of Human Mesenchymal Stem Cells Is Dependent on Vimentin Intermediate Filaments
Poonam Sharma1, Zachary T Bolten1, Diane R Wagner2
1Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Abstract:
This erratum is to correct the following: (1) in the Western Blotting subsection under the Materials and Methods section, the concentration of protein from each sample loaded into Criterion Tris-HCl gels was incorrectly stated as 155 µg of protein. The correct value is 9.7 µg; (2) in Fig. 1b, the bar graph showed incorrect values for semi-quantitation of Western blots. Figure 1 has been updated with a corrected graph in Fig. 1b only.
Insights
This scientific erratum corrects protein loading amounts in Western blotting methods and updates semi-quantitation data in Figure 1b. These corrections ensure accurate reporting of experimental results in molecular biology research.
Area of Science:
- Molecular Biology
- Biochemistry
Background:
- Accurate quantification is crucial for reproducible Western blotting.
- Previous reporting contained errors in protein loading concentration and data representation.
Purpose of the Study:
- To correct inaccuracies in the Materials and Methods section regarding protein loading.
- To rectify erroneous data presented in Figure 1b for Western blot semi-quantitation.
Main Methods:
- Correction of protein concentration values used in Western blotting.
- Updating of a specific bar graph (Figure 1b) to reflect accurate semi-quantitation data.
Main Results:
- The protein concentration loaded per sample was corrected from 155 µg to 9.7 µg.
- Figure 1b has been revised to display accurate semi-quantitation values for Western blots.
Conclusions:
- The erratum ensures the integrity and accuracy of the published experimental data.
- Corrected data facilitates reliable interpretation of Western blot results.
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