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Updated: Feb 16, 2026

Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
Inhibitory effect of resin composite containing S-PRG filler on Streptococcus mutans glucose metabolism
Haruaki Kitagawa1, Saeki Miki-Oka2, Gen Mayanagi3
1Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Objectives:
Resin composites containing surface pre-reacted glass-ionomer (S-PRG) fillers have been reported to inhibit Streptococcus mutans growth on their surfaces, and their inhibitory effects were attributed to BO33- and F- ions. The aim of this study was to evaluate S. mutans acid production through glucose metabolism on resin composite containing S-PRG fillers and assess inhibitory effects of BO33- and F- on S. mutans metabolic activities.
Methods:
The pH change through S. mutans acid production on experimental resin composite was periodically measured after the addition of glucose. Inhibitory effects of BO33- or F- solutions on S. mutans metabolism were evaluated by XTT assays and measurement of the acid production rate.
Results:
The pH of experimental resin containing S-PRG fillers was significantly higher than that of control resin containing silica fillers (p < 0.05). OD450 values by XTT assays and S. mutans acid production rates significantly decreased in the presence of BO33- and F- compared with the absence of these ions (p < 0.05).
Conclusions:
pH reduction by S. mutans acid production was inhibited on resin composite containing S-PRG fillers. Moreover, S. mutans glucose metabolism and acid production were inhibited in the presence of low concentrations of BO33- or F-.
Clinical Significance:
BO33- or F- released from resin composite containing S-PRG fillers exhibits inhibitory effects on S. mutans metabolism at concentrations lower than those which inhibit bacterial growth.
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