Related Experiment Video
Updated: Feb 16, 2026
09:51
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
11.2K
Tsc1-dependent transcriptional programming of dendritic cell homeostasis and function
Yuechen Luo1, Jingru Liu2, Xiaolei Sun1
1State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Experimental Cell Research
|January 3, 2018
Summary
Tuberous sclerosis complex 1 (Tsc1) regulates dendritic cell (DC) function by controlling gene expression. This study identifies Tsc1-regulated genes crucial for DC survival, proliferation, metabolism, and antigen presentation, partially via mTORC1 inhibition.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Dendritic cells (DCs) are critical for initiating adaptive immune responses.
- Tuberous sclerosis complex 1 (Tsc1) plays a role in DC development and activation.
- Tsc1 in DCs influences T-cell homeostasis and response, partly by inhibiting mTORC1.
Purpose of the Study:
- To elucidate the molecular mechanisms of transcriptional regulation by Tsc1 in DCs.
- To identify genes and pathways controlled by Tsc1 in dendritic cells.
- To understand how Tsc1 impacts DC homeostasis and function at the transcriptional level.
Main Methods:
- Comparative transcriptional profiling of Tsc1-deficient DCs versus wild-type DCs.
- Analysis of gene sets involved in DC survival, proliferation, metabolism, and antigen presentation.
Main Results:
- Tsc1 globally regulates specific gene sets critical for DC homeostasis and function.
- Tsc1 influences DC survival, proliferation, metabolism, and antigen presentation pathways.
- The effect of Tsc1 on DC gene expression is partially dependent on mTORC1 inhibition.
Conclusions:
- Tsc1 is a key regulator of dendritic cell transcriptional programming.
- This study provides a comprehensive molecular basis for Tsc1's role in DC homeostasis and function.
- Understanding Tsc1's transcriptional control offers insights into adaptive immunity regulation.
Related Concept Videos
01:16What is Homeostasis?
57.6K
Maintaining homeostasis requires that the body continuously maintain its internal conditions. Each physiological condition has a particular set point, from body temperature to blood pressure to levels of certain nutrients. A set point is the physiological value around which the normal range fluctuates. A normal range is a restricted set of values that is optimally healthful and stable. For example, the set point for normal human body temperature is approximately 37°C (98.6°F).
57.6K
01:31pH Homeostasis
20.2K
Acid-base homeostasis is essential for maintaining normal physiological activities in humans. The pH of various body fluids is strictly regulated because it is critical for the optimal activity of enzymes involved in metabolic reactions. Enzymes are basically proteins, so, any significant change in pH can affect their structure and activity. In humans, pH is regulated using three primary mechanisms— chemical buffer systems, respiratory regulation, and renal regulation.
Respiratory...
Respiratory...
20.2K
02:16Transcription Factors
82.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.9K
01:10Transcription
157.3K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.3K
02:35Transcription Elongation Factors
14.1K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.1K
02:23Master Transcription Regulators
7.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.9K