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The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide
Published on: March 19, 2018
Effect of pretreatments on isolation of bioactive polysaccharides from spent coffee grounds using subcritical water
Adane Tilahun Getachew1, Yeon Jin Cho2, Byung Soo Chun2
1Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Korea; School of Chemical and Bioengineering, Addis Ababa Institute of Technology, Addis Ababa University, P.O. Box 385, Addis Ababa, Ethiopia.
Abstract:
In this study, we used a novel approach to recover polysaccharide from spent coffee ground (SCG) by combining pretreatments and subcritical water hydrolysis (SCWH). The independent variables which affect SCWH were optimized using response surface methodology. The highest yield of SCG polysaccharides (SCGPSs) (18.25 ± 0.21%) was obtained using ultrasonic pretreatment and SCWH conditions of temperature (178.85 °C), pressure (20 bar), and extraction time (5 min). The extracted SCGPSs showed high antioxidant activity as measured using ABTS+ and DPPH radical scavenging assay with IC50 values of 1.83 ± 0.03 and 2.66 ± 0.13 mg/ml respectively. SCGPSs also showed in vitro hypoglycemic activities. The structural and thermal characterization of the polysaccharide showed that the extracted polysaccharide has a typical carbohydrate features. The results of this study suggested that the extracted polysaccharide could have a potential application in food and related industries.
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