Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An in vitro bleeding time test.

D B Brubaker1

  • 1Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.

American Journal of Clinical Pathology
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

A novel Platelet-Stat test offers a reproducible method for measuring in vitro bleeding time. This new device provides greater precision compared to traditional in vivo bleeding time tests.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro bleeding time test can diagnose thrombotic thrombocytopenia purpura and can possibly monitor therapeutic plasma apheresis.

Journal of clinical apheresis·2000
Same author

Intravascular and total body platelet equilibrium in healthy volunteers and in thrombocytopenic patients transfused with single donor platelets.

American journal of hematology·1998
Same author

Posttransfusion graft-versus-host disease in immunocompetent patients.

Transfusion·1994
Same author

Correction of the corrected count increment units.

Transfusion·1993
Same author

Immunopathogenic mechanisms of posttransfusion graft-vs-host disease.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·1993
Same author

The function of elevated plasma fibronectin in preeclampsia.

American journal of obstetrics and gynecology·1992

Area of Science:

  • Hematology
  • Clinical Diagnostics
  • Medical Device Development

Background:

  • Assessing bleeding time is crucial for diagnosing and managing hemostatic disorders.
  • Existing in vivo bleeding time tests can lack precision and reproducibility.
  • A need exists for a more accurate and reliable in vitro method to measure bleeding time.

Purpose of the Study:

  • To introduce and validate a new conical plastic device and method, the Platelet-Stat test, for measuring in vitro bleeding time.
  • To evaluate the test's performance with different anticoagulants.
  • To assess the test's precision and its ability to detect changes in bleeding time, such as after aspirin administration.

Main Methods:

  • Developed a novel conical plastic device and method termed the Platelet-Stat test.

Related Experiment Videos

  • Utilized 10 mL of citrated blood collected via venipuncture for the in vitro bleeding time assay.
  • Evaluated anticoagulant performance, focusing on citrate, and assessed within-run precision (mean time 39 +/- 6.7 seconds, CV 17%).
  • Conducted an aspirin study on eight volunteers, measuring bleeding times pre- and post-aspirin administration (18-24 hours).

    • Main Results:

    • The Platelet-Stat test demonstrated a mean bleeding time of less than 1 minute in eight volunteers.
    • Citrate was identified as the optimal anticoagulant for the test.
    • The test showed high reproducibility, with a within-run coefficient of variation of 17%.
    • Post-aspirin administration, in vitro bleeding times significantly increased to over 7 minutes, compared to pre-aspirin times of less than 1 minute.

    Conclusions:

    • The Platelet-Stat test is a reproducible and precise in vitro method for measuring bleeding time.
    • The test effectively detects alterations in hemostasis, as demonstrated by the aspirin study.
    • This novel device offers a more precise alternative to traditional in vivo bleeding time assessments.