Related Experiment Video

Updated: Feb 16, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.1K

Regulation of the Mdm2-p53 pathway by the ubiquitin E3 ligase MARCH7

Kailiang Zhao1, Yang Yang1, Guang Zhang1

  • 1CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, Anhui, China.

EMBO Reports
|January 4, 2018
PubMed

Insights

The E3 ligase MARCH7 stabilizes Mdm2 by preventing its degradation. This stabilization enhances Mdm2

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Biochemistry

Background:

  • The tumor suppressor p53 is crucial for cancer prevention.
  • Mdm2, a ubiquitin E3 ligase, regulates p53 stability and activity.
  • The regulatory mechanisms of Mdm2 are not fully understood.

Purpose of the Study:

  • To investigate the role of MARCH7 in regulating Mdm2.
  • To elucidate the mechanism by which MARCH7 affects the Mdm2-p53 pathway.

Main Methods:

  • Co-immunoprecipitation to assess protein interactions.
  • Ubiquitination assays to determine polyubiquitination patterns.
  • Western blotting to evaluate protein stability.
  • Cell proliferation and apoptosis assays.

Main Results:

  • MARCH7 physically interacts with Mdm2.
  • MARCH7 catalyzes Lys63-linked polyubiquitination of Mdm2, stabilizing it.
  • MARCH7 promotes Mdm2-dependent degradation of p53.
  • MARCH7 influences cell proliferation, apoptosis, and tumorigenesis via p53.

Conclusions:

  • MARCH7 is a novel regulator of Mdm2 stability.
  • MARCH7 plays a significant role in the Mdm2-p53 pathway.
  • MARCH7 impacts cancer-related cellular processes through p53 regulation.
Keywords:
MARCH7Mdm2p53ubiquitination

More Related Videos

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

9.4K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.6K
GTPases and their Regulation02:14

GTPases and their Regulation

Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.9K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.4K