Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

32.8K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.8K
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

7.8K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
7.8K
Framing Effects03:26

Framing Effects

8.0K
Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
8.0K
Frames01:30

Frames

850
Frames are essential components of various mechanical and structural systems used daily. These structures are known for their stability and ability to bear heavy loads. A frame is constructed using two-force and multi-force members, interconnected using pin joints. In contrast, trusses are made entirely of two-force members.
Frames are versatile and widely used in various applications such as structural supports for beams and columns, automobile chassis construction, and in the construction...
850
Frames: Problem Solving II01:26

Frames: Problem Solving II

509
Consider a hydraulic hoist supporting a load of 1 kN. Assuming a simplified schematic representation of this frame structure, the force acting on BD and BF members can be determined.
509
Frames: Problem Solving I01:24

Frames: Problem Solving I

992
Consider a jib crane with an external load suspended from the pulley. The dimensions of the crane members are shown in the figure. A systematic analysis of the frame structure is required to determine the reaction forces at the pin joints, assuming that the pulleys are frictionless.
992

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacteria producing the bioplastic polyhydroxybutyrate kill the nematode Caenorhabditis elegans.

PLoS biology·2026
Same author

Glycolytic Specialization Shapes Neuronal Physiology and Function <i>in vivo</i>.

bioRxiv : the preprint server for biology·2026
Same author

A systems-level, semi-quantitative landscape of metabolic flux in C. elegans.

Nature·2025
Same author

Systems-level design principles of metabolic rewiring in an animal.

Nature·2025
Same author

Worm Perturb-Seq: massively parallel whole-animal RNAi and RNA-seq.

bioRxiv : the preprint server for biology·2025
Same author

Enhanced flux potential analysis links changes in enzyme expression to metabolic flux.

Molecular systems biology·2025
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Feb 16, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

869

Generating an Open Reading Frame (ORF) Entry Clone and Destination Clone.

John S Reece-Hoyes, Albertha J M Walhout

    Cold Spring Harbor Protocols
    |January 4, 2018
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details using the Gateway recombinatorial cloning system to create entry and destination clones. This method facilitates efficient cloning of open reading frames (ORFs) and other DNA fragments for various applications.

    More Related Videos

    CRISPR Guide RNA Cloning for Mammalian Systems
    06:48

    CRISPR Guide RNA Cloning for Mammalian Systems

    Published on: October 2, 2018

    73.1K
    A Customizable Protocol for String Assembly gRNA Cloning STAgR
    10:00

    A Customizable Protocol for String Assembly gRNA Cloning STAgR

    Published on: December 26, 2018

    10.2K

    Related Experiment Videos

    Last Updated: Feb 16, 2026

    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
    06:28

    Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

    Published on: September 2, 2025

    869
    CRISPR Guide RNA Cloning for Mammalian Systems
    06:48

    CRISPR Guide RNA Cloning for Mammalian Systems

    Published on: October 2, 2018

    73.1K
    A Customizable Protocol for String Assembly gRNA Cloning STAgR
    10:00

    A Customizable Protocol for String Assembly gRNA Cloning STAgR

    Published on: December 26, 2018

    10.2K

    Area of Science:

    • Molecular Biology
    • Recombinant DNA Technology

    Background:

    • The Gateway recombinatorial cloning system offers a versatile method for DNA cloning.
    • Efficiently transferring DNA fragments, such as open reading frames (ORFs), between vectors is crucial for molecular biology applications.

    Purpose of the Study:

    • To describe a protocol for creating entry and destination clones using the Gateway system.
    • To provide a guide for cloning various DNA fragments, including ORFs, promoter sequences, and 3' untranslated regions (UTRs).

    Main Methods:

    • Utilizing BP recombination to clone an ORF from a cDNA source into the Donor vector pDONR 221.
    • Transferring the ORF from the entry clone into the Destination vector pDEST-15 for expression as an amino-terminal GST-fusion protein.

    Main Results:

    • Successful creation of an entry clone containing the ORF.
    • Generation of a destination clone expressing the ORF as a GST-fusion protein.
    • The protocol requires 9-15 days for construction and transformation, excluding sequence confirmation.

    Conclusions:

    • The described Gateway cloning protocol provides a reliable method for generating entry and destination clones.
    • This technique is adaptable for cloning diverse DNA fragments, enabling flexible genetic engineering strategies.
    • The system facilitates the expression of cloned DNA fragments, such as ORFs, as fusion proteins.