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MARCH8 is associated with poor prognosis in non-small cell lung cancers patients

Jiye Fan1,2, Liying Tian2, Manli Li1

  • 1Department of Biochemistry and Molecular Biology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, PR China.

Oncotarget
|January 4, 2018
PubMed

Insights

Membrane-associated RING-CH (MARCH) ligase 8 (MARCH8) is downregulated in non-small cell lung cancer (NSCLC). Overexpressing MARCH8 inhibits NSCLC progression by affecting cell proliferation, metastasis, and apoptosis via the PI3K/mTOR pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MARCH8 is a ubiquitin ligase family member with an unknown mechanism of action.
  • Understanding MARCH8's role in non-small cell lung cancer (NSCLC) is crucial for therapeutic development.

Purpose of the Study:

  • To investigate MARCH8 expression in NSCLC tissues.
  • To analyze the correlation between MARCH8 and NSCLC patient characteristics and prognosis.
  • To explore the functional roles and molecular mechanisms of MARCH8 in NSCLC progression.

Main Methods:

  • MARCH8 protein and mRNA expression analysis in NSCLC samples and TCGA database.
  • Correlation analysis between MARCH8 expression and NSCLC clinical characteristics/prognosis.
  • Ectopic expression, western blot, and cell apoptosis assays to investigate MARCH8 function in NSCLC cell lines (A549, H1299).

Main Results:

  • MARCH8 was significantly downregulated in NSCLC tissues compared to adjacent normal tissues.
  • Overexpression of MARCH8 suppressed NSCLC cell proliferation and metastasis.
  • MARCH8 overexpression enhanced apoptosis in NSCLC cells, potentially through the PI3K and mTOR signaling pathways.

Conclusions:

  • MARCH8 plays a critical role in suppressing NSCLC carcinogenesis and progression.
  • MARCH8 may serve as a valuable predictive biomarker for NSCLC.
  • MARCH8 represents a potential therapeutic target for NSCLC treatment.
Keywords:
MARCH8NSCLCapoptosisproliferationubiquitin ligase

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