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Phenotypic and genotypic characterization of macrolide resistance among Staphylococcus aureus isolates in Isfahan,

Hossein Sedaghat1, Bahram Nasr Esfahani1, Sina Mobasherizadeh2

  • 1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Iranian Journal of Microbiology
|January 4, 2018
PubMed
Abstract

Insights

Macrolide resistance in Staphylococcus aureus is increasing, with the constitutive MLSB phenotype and ermC gene being most common. This study highlights expanding erythromycin resistance mechanisms in Isfahan.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Macrolide, lincosamide, and streptogramin B (MLSB) antibiotics are crucial for treating *Staphylococcus aureus* infections.
  • Understanding resistance patterns is vital for effective treatment strategies.

Purpose of the Study:

  • To characterize the phenotypic and genotypic macrolide resistance in *S. aureus* isolates.
  • To investigate resistance in clinical samples and nasal swabs from patients and healthcare workers.

Main Methods:

  • Collected 162 non-duplicate *S. aureus* isolates from clinical samples and nasal swabs.
  • Determined antibiotic resistance profiles using disk diffusion tests.
  • Detected resistance genes using PCR.

Main Results:

  • 43.8% of isolates were erythromycin-resistant, and 34% were methicillin-resistant *S. aureus* (MRSA).
  • The most prevalent phenotype was constitutive MLSB (cMLSB) at 32%.
  • The most common resistance genes were *erm*C (35.2%), *erm*A (20.4%), and *msr*A (17.3%).

Conclusions:

  • Constitutive MLSB phenotype and the *erm*C gene are the most frequent erythromycin resistance markers.
  • The study indicates expanding erythromycin resistance mechanisms in *S. aureus* in Isfahan.
Keywords:
D-testInducible resistanceStaphylococcus aureuserm AermCmsrA

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