Related Experiment Video

Updated: Feb 16, 2026

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

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CD86 regulates myeloma cell survival

Catherine M Gavile1, Benjamin G Barwick1, Scott Newman2

  • 1Department of Hematology and Oncology, Winship Cancer Institute, Emory University, Atlanta, GA.

Blood Advances
|January 4, 2018
PubMed

Insights

Blocking the CD28-CD86 pathway induces death in multiple myeloma cells. This approach downregulates key survival factors, offering a promising new therapeutic strategy for this plasma cell malignancy.

Area of Science:

  • Immunology
  • Oncology
  • Molecular Biology

Background:

  • Multiple myeloma, a plasma cell malignancy, has seen improved prognoses but still requires novel therapeutic strategies for a potential cure.
  • The CD28-CD86 co-stimulatory pathway plays a role in immune regulation and cell survival.

Purpose of the Study:

  • To investigate the therapeutic potential of targeting the CD28-CD86 pathway in multiple myeloma.
  • To elucidate the mechanisms by which this pathway influences myeloma cell viability.

Main Methods:

  • Silencing of CD28 or CD86 genes in myeloma cells.
  • Analysis of downstream effects on integrin and IRF4 expression.
  • Assessment of cell death induction and survival factor modulation.

Main Results:

  • Silencing CD28 or CD86 effectively induced myeloma cell death.
  • Inhibition of the CD28-CD86 pathway led to downregulation of integrins and IRF4, a crucial myeloma survival factor.
  • The prosurvival activity of CD86 was found to be dependent on its cytosolic domain.

Conclusions:

  • Targeting the CD28-CD86 pathway represents a promising therapeutic strategy for multiple myeloma.
  • Modulation of this pathway impacts critical cellular processes involved in myeloma cell viability, suggesting its potential as a novel treatment avenue.

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