Related Experiment Video

Updated: Feb 16, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
06:59

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

10.9K

Exosomal small RNA sequencing uncovers the microRNA dose markers for power frequency electromagnetic field exposure

Hualiang Li1, Lin Lin2, Li Li1

  • 1a Electric Power Research Institute of Guangdong Power Grid , Guangzhou , PR China.

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
|January 4, 2018
PubMed
Abstract

Insights

Power frequency electromagnetic field (PFEMF) exposure poses health risks, but dose determination is difficult. This study identified specific serum exosomal microRNAs (miRNAs) as biomarkers to accurately assess PFEMF exposure levels in mice.

Area of Science:

  • Biomedical Science
  • Environmental Health
  • Molecular Biology

Background:

  • Power frequency electromagnetic field (PFEMF) exposure is a growing public health concern.
  • Accurate diagnosis and prognosis of PFEMF exposure effects are challenging due to difficulties in determining precise exposure doses.
  • Biomarkers are needed to quantify PFEMF exposure and its health implications.

Purpose of the Study:

  • To identify reliable biomarkers for determining the exact dose of PFEMF exposure.
  • To investigate the potential health risks associated with different PFEMF exposure levels.
  • To establish a foundation for future clinical interventions related to PFEMF exposure.

Main Methods:

  • Mice were exposed to varying doses of PFEMF.
  • Serum exosomes were isolated, and microRNAs (miRNAs) were extracted and sequenced.
  • Bioinformatic analysis, including Gene Ontology and KEGG pathway analysis, and qRT-PCR were used to identify and validate miRNA biomarkers.

Main Results:

  • Specific serum exosomal miRNAs were identified as significant biomarkers for different PFEMF exposure doses (0.1, 0.5, 2.5 mT) and common exposure.
  • Pathway analysis indicated that these miRNA markers are involved in crucial neural system pathophysiological processes.
  • The identified markers are predicted to play roles in cancer and other disease-related signaling pathways.

Conclusions:

  • Aberrantly expressed serum exosomal miRNAs, such as miR-128-3p, miR-133a-3p, miR-142a-5p, miR-218-5p, and miR-199a-3p, serve as informative markers for PFEMF exposure.
  • These miRNA biomarkers can help identify the exact dose of PFEMF exposure.
  • The findings provide a basis for future clinical interventions aimed at mitigating PFEMF-related health risks.
Keywords:
Power frequency electromagnetic fieldsexosomemiR-128-3pmiR-133a-3pmiR-142a-5pmiR-199a-3pmiR-218-5pmicroRNA

More Related Videos

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

12.3K
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

32.4K

Related Concept Videos

Electromagnetic Fields01:30

Electromagnetic Fields

Electric fields generated by static charges, often referred to as electrostatic fields, are characteristically different from electric fields created by time-varying magnetic fields. While the former is a conservative field, implying that no net work is done on a test charge if it goes around in a complete loop in the field, the latter is, by definition, not a conservative field; net work is done, and it is proportional to the rate of change of magnetic flux.
However, the observation of...
2.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.1K
The Electromagnetic Spectrum02:37

The Electromagnetic Spectrum

The electromagnetic spectrum consists of all the types of electromagnetic radiation arranged according to their frequency and wavelength. Each of the various colors of visible light has specific frequencies and wavelengths associated with them, and you can see that visible light makes up only a small portion of the electromagnetic spectrum. Because the technologies developed to work in various parts of the electromagnetic spectrum are different, for reasons of convenience and historical...
65.7K
The Electromagnetic Spectrum01:24

The Electromagnetic Spectrum

Electromagnetic waves are categorized according to their wavelengths and frequencies, giving the electromagnetic spectrum. These waves are classified as radio, infrared, ultraviolet, etc. Radio waves refer to electromagnetic radiation with wavelengths ranging from millimeters to kilometers. Radio waves are commonly used for audio communications (i.e., radios) and typically result from an alternating current in the wires of a broadcast antenna. They cover a broad wavelength range and are used...
33.9K
Dose Size and Dosing Frequency: Determination Methods01:21

Dose Size and Dosing Frequency: Determination Methods

Determining the optimal dose size and dosing frequency in pharmacotherapy is crucial for achieving therapeutic effectiveness while minimizing adverse effects. This article explores the methodologies employed in determining these parameters, focusing on their significance and interplay to tailor dosing regimens.Dose Size: Dose size refers to the amount of a drug administered in a single dose. It is determined based on the drug's pharmacodynamics and pharmacokinetics properties and...
345