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One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
Published on: October 23, 2017
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Fluorescence of radiation-induced tissue damage
Irina Raznitsyna1,2, Polina Kulikova1, Dmitry Rogatkin1
1a Laboratory of Medical and Physics Research , Moscow Regional Research and Clinical Institute (MONIKI) , Moscow , Russian Federation.
International Journal of Radiation Biology
|January 4, 2018
Summary
Enhanced photosensitizer fluorescence in radiation-damaged mouse limbs indicates inflammation. This finding may impact interpreting intraoperative navigation in previously irradiated tumors and guide radiology dose fractionation.
Area of Science:
- Medical physics
- Radiology
- Biomedical optics
Background:
- Radiation therapy can cause tissue damage and inflammation.
- Photosensitizers are used in various medical applications, including diagnostics and therapeutics.
- Monitoring treatment-induced changes in vivo is crucial for patient management.
Purpose of the Study:
- To evaluate in vivo changes in photosensitizer fluorescence within radiation-induced damage areas.
- To compare fluorescence in damaged versus intact tissue areas.
- To correlate fluorescence changes with blood parameters and histological data.
Main Methods:
- White outbred SHK mice (n=21) received intraperitoneal injections of the photosensitizer 'Photosens'.
- Right hindlimbs were irradiated with 25 Gy.
- In vivo fluorescence intensity was monitored for seven weeks using a laser diagnostic system.
- Histological examination and blood tests were performed concurrently.
Main Results:
- Significantly increased laser-induced fluorescence of 'Photosens' was observed in radiation-damaged areas compared to intact contralateral limbs.
- Laboratory blood tests and histological findings suggested the presence of inflammation in the damaged tissues.
- Exogenous fluorescence enhancement correlated with radiation-induced inflammation in noncancerous tissues.
Conclusions:
- Enhanced exogenous photosensitizer fluorescence is detectable in radiation-induced inflammation.
- These findings may influence the interpretation of intraoperative navigation in previously irradiated tumor sites.
- The results could aid in developing individualized dose fractionation algorithms in radiology.
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