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Increased expression of PRKCB mRNA in peripheral blood mononuclear cells from patients with systemic lupus

Zhengwei Zhu1, Lulu Yang1, Yaohua Zhang2

  • 1Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, Hefei, Anhui, China.

Annals of Human Genetics
|January 4, 2018
PubMed

Insights

Increased PRKCB mRNA expression is linked to systemic lupus erythematosus (SLE), but negatively correlates with disease activity. This suggests PRKCB mRNA

Area of Science:

  • Immunology
  • Genetics
  • Molecular Biology

Background:

  • Previous studies linked PRKCB gene polymorphism to systemic lupus erythematosus (SLE).
  • The role of PRKCB mRNA expression in SLE pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the relationship between PRKCB mRNA expression and SLE disease activity (SLEDAI) and clinical manifestations.
  • To explore the association between the PRKCB rs16972959 polymorphism and PRKCB mRNA expression levels in SLE patients.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (RT-PCR) to measure PRKCB mRNA levels in 60 SLE patients and 62 controls.
  • Sequenom MassArray System to genotype the PRKCB rs16972959 single nucleotide polymorphism (SNP).

Main Results:

  • PRKCB mRNA expression was significantly higher in SLE patients than in controls (P < 0.001).
  • PRKCB mRNA levels showed a negative correlation with SLEDAI (r = -0.322, P < 0.05), indicating lower expression with higher disease activity.
  • Lower PRKCB mRNA expression was associated with rash and proteinuria in SLE patients.
  • The rs16972959 SNP was an expression quantitative trait locus (eQTL) for PRKCB, with the SLE risk allele increasing expression in monocytes.
  • The rs16972959 SNP showed a suggestive association with vasculitis in SLE (P = 0.00718).

Conclusions:

  • PRKCB mRNA expression may play a role in the pathogenesis of SLE.
  • Further research is needed to elucidate the precise role of PRKCB in SLE development and progression.
Keywords:
PRKCBperipheral blood mononuclear cellsquantitative reverse transcription polymerase chain reactionsingle nucleotide polymorphismsystemic lupus erythematosus

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