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Near-Normalized Gene Expression Profiles in Bladder With Detrusor Overactivity in Rats With Bladder Outlet
Tack Lee1, U-Sung Lim1, Dong Hyuk Kang1
1Department of Urology, Inha University College of Medicine, Incheon, Korea.
International Neurourology Journal
|January 5, 2018
Summary
Detrusor overactivity (DO) in bladder outlet obstruction (BOO) may play a protective role. Bladder function and gene expression normalized in rats with DO after deobstruction, suggesting adaptive neuroplasticity.
Area of Science:
- Urology
- Molecular Biology
- Physiology
Background:
- Detrusor overactivity (DO) is common in bladder diseases but its pathophysiological role is unclear.
- DO can persist after bladder outlet obstruction (BOO) and deobstruction, offering a model to study molecular changes.
Purpose of the Study:
- To investigate the molecular effects of persistent DO following BOO induction and subsequent deobstruction.
- To understand the role of DO in bladder adaptation to obstruction and relief.
Main Methods:
- Awake cystometry was performed on female Sprague-Dawley rats in sham, BOO, deobstructed with DO (DDO), and deobstructed without DO (non-DDO) groups.
- Gene expression profiles were analyzed using RNA extraction and comparison between sham and model groups.
Main Results:
- DO was observed in 83% of BOO rats and 46% of deobstructed rats.
- The non-DDO group had significantly greater residual volume than the DDO group.
- Gene expression analysis revealed significant differences: BOO group had 898 upregulated/2,911 downregulated genes; non-DDO group had 3,472 upregulated/4,025 downregulated genes; DDO group had only 145 upregulated/72 downregulated genes.
Conclusions:
- Bladder function and gene expression normalized in deobstructed rats with DO, unlike those without DO.
- Persistent DO after BOO and deobstruction may indicate a protective, adaptive neuroplastic response to bladder stress.
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