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Complete genome sequence of esterase-producing bacterium Croceicoccus marinus E4A9T
Yue-Hong Wu1, Hong Cheng1, Ying-Yi Huo1
1Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, 36th North BaoChu Road, Hangzhou, 310012 China.
Standards in Genomic Sciences
|January 5, 2018
Summary
Deep-sea bacteria Croceicoccus marinus E4A9 produces esterases, enzymes valuable in various industries. Its genome sequencing reveals 10 esterase genes, with three successfully expressed for potential biotechnological applications.
Area of Science:
- Marine Microbiology
- Genomics
- Biotechnology
Background:
- Deep-sea environments harbor unique microbial life with potential industrial applications.
- Croceicoccus marinus E4A9 was isolated from East Pacific polymetallic nodule sediment.
- This bacterium produces esterases, enzymes with broad industrial utility.
Observation:
- The genome of C. marinus E4A9 comprises approximately 4.1 million base pairs, including a chromosome and two plasmids.
- The genome contains 3653 coding sequences, 48 tRNAs, and rRNA gene operons.
- Ten esterase genes were identified in the C. marinus E4A9 genome.
Findings:
- Three specific esterases (E3, E6, E10) were successfully cloned and expressed in Escherichia coli.
- The expressed esterases were found in a soluble form, indicating successful protein folding.
- The genome analysis provides insights into the metabolic pathways and environmental adaptations of C. marinus E4A9.
Implications:
- The successful expression of C. marinus E4A9 esterases highlights their potential for biotechnological applications.
- This research provides a foundational genomic resource for future studies on marine esterase enzymes.
- Understanding the metabolic capabilities of C. marinus E4A9 can inform its use in industrial processes.
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