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Updated: Feb 16, 2026

Highly-Multiplexed Tissue Imaging with Raman Dyes
Published on: April 21, 2022
A highly fluorescent water soluble spirobifluorene dye with a large Stokes shift: synthesis, characterization and
Friederike Schlüter1, Kristina Riehemann, Nermin Seda Kehr
1Organisch-Chemisches Institut and CeNTech, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster, Germany.
Abstract:
The first water-soluble spirobifluorene derivative has been synthesized, which exhibits high fluorescence quantum yield and a large Stokes shift (>100 nm). Proteins induce changes in the emission color, allowing to reach the nanomolar detection limit. Cellular uptake and cytotoxicity studies in living cells revealed its biocompatibility, indicating potential application for live cell imaging.
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