A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS₂ Nanosheet-Supported Host-Guest...

Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms. However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG. Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition. Multitypes of host molecule modified MoS₂ nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing. By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously. The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.