Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diazepam--morphine hypnotic synergism in rats.

I Kissin1, P T Brown, E L Bradley

  • 1Department of Anesthesiology, School of Medicine, University of Alabama, Birmingham.

Anesthesiology
|April 1, 1989
PubMed
Summary

The combination of diazepam and morphine showed a synergistic effect on the righting reflex in rats, indicating an enhanced interaction. This synergistic effect is unlikely to be due to how the drugs are processed by the body.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcervical endometrial biopsy in the baboon: Effects on the menstrual cycle and relations to protein and dry matter content and histology.

American journal of primatology·2020
Same author

Cellular conditions that modulate the fungicidal activity of occidiofungin.

Journal of applied microbiology·2017
Same author

Fenton's reagent for the rapid and efficient isolation of microplastics from wastewater.

Chemical communications (Cambridge, England)·2016
Same author

Feasibility of a rural palliative supportive service.

Rural and remote health·2015
Same author

Model studies of migration from paper and board into fruit and vegetables and into Tenax as a food simulant.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2014
Same author

Printing ink compounds in foods: UK survey results.

Food additives & contaminants. Part B, Surveillance·2014

Area of Science:

  • Pharmacology
  • Neuroscience
  • Drug Interaction Studies

Background:

  • The righting reflex is a crucial indicator of central nervous system depression.
  • Understanding drug interactions is vital for safe and effective therapeutic use.

Purpose of the Study:

  • To investigate the interaction between diazepam and morphine on the righting reflex in rats.
  • To determine if the combination exhibits synergistic, additive, or antagonistic effects.
  • To explore the pharmacokinetic and pharmacodynamic basis of the observed interaction.

Main Methods:

  • Determination of median effective doses (ED50) for diazepam and morphine alone and in combination using probit analysis.
  • Measurement of brain drug concentrations via radioimmunoassay following equieffective doses.

Related Experiment Videos

  • Comparison of equieffective doses and brain concentrations using fractional and isobolographic analyses.

    • Main Results:

    • The diazepam-morphine combination demonstrated a synergistic interaction on the righting reflex.
    • Fractional and isobolographic analyses confirmed the synergistic nature of the interaction.
    • The observed synergy is unlikely to be explained by pharmacokinetic factors.

    Conclusions:

    • Diazepam and morphine exhibit a synergistic interaction, leading to a potentiated effect on the righting reflex in rats.
    • The interaction is likely pharmacodynamic rather than pharmacokinetic.
    • These findings have implications for understanding combined CNS depressant effects.