Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.2K
Elements and Compounds01:27

Elements and Compounds

105.5K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
105.5K
Periodic Classification of the Elements04:00

Periodic Classification of the Elements

60.5K
The periodic table arranges atoms based on increasing atomic number so that elements with the same chemical properties recur periodically. When their electron configurations are added to the table, a periodic recurrence of similar electron configurations in the outer shells of these elements is observed. Because they are in the outer shells of an atom, valence electrons play the most important role in chemical reactions. The outer electrons have the highest energy of the electrons in an atom...
60.5K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

24.4K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.4K
Classification of Elements and Compounds02:54

Classification of Elements and Compounds

73.9K
Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond. Elements are classified as atomic or molecular based on the nature of their basic units.
Compounds are pure substances composed of two or more elements in fixed, definite proportions. Compounds are classified as ionic or molecular (covalent) based on the bonds...
73.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gigabase-scale deletion scanning of the human genome.

bioRxiv : the preprint server for biology·2026
Same author

SciPhy: A Bayesian phylogenetic framework using sequential genetic lineage tracing data.

Nature communications·2026
Same author

Designed Minibinders Rewire Receptor Signaling to Enable Functional Human Myogenic Reprogramming.

bioRxiv : the preprint server for biology·2026
Same author

Retracing and rewriting the evolutionary trajectories of mammalian developmental enhancers.

bioRxiv : the preprint server for biology·2026
Same author

Multi-scale dissection, compaction and derivatization of mammalian developmental enhancers.

bioRxiv : the preprint server for biology·2026
Same author

Technical and biological sources of noise confound multiplexed enhancer AAV screening.

Nature communications·2026
Same journal

Inositol Thiophosphates as Inhibitors of Mammalian, Plant, and Fungal Phytases.

ACS chemical biology·2026
Same journal

Synthesis and Characterization of the Spectroscopic and Imaging Utilities of Two Indole-Based Cyan Fluorescent Nucleoside Analogues.

ACS chemical biology·2026
Same journal

Indole Ring Expansion and Rearrangement-Enabled Quinoline Scaffold Formation in the Biosynthesis of the Antitumor Monoterpene Indole Alkaloid Camptothecin.

ACS chemical biology·2026
Same journal

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

ACS chemical biology·2026
Same journal

Development of Next-Generation Fluoroacetamidine-Containing Activity-Based Probes for the Selective Labeling of the Protein Arginine Deiminases (PADs).

ACS chemical biology·2026
Same journal

Spectroelectrochemical Insight into Reaction Mechanisms of Cell-Penetrating Peptides on Charged Membrane Surfaces.

ACS chemical biology·2026
See all related articles

Related Experiment Video

Updated: Feb 16, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.3K

Identifying Novel Enhancer Elements with CRISPR-Based Screens.

Jason C Klein1, Wei Chen2, Molly Gasperini1

  • 1Department of Genome Sciences, University of Washington , Seattle, Washington 98195, United States.

ACS Chemical Biology
|January 5, 2018
PubMed
Summary
This summary is machine-generated.

CRISPR genome editing tools now allow researchers to study gene enhancers within their natural genomic context. This approach enables precise mutation and epigenetic modulation for a deeper understanding of development.

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.8K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.8K

Related Experiment Videos

Last Updated: Feb 16, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.3K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.8K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.8K

Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Gene enhancers regulate gene expression crucial for development.
  • Historically, enhancers were studied outside their native genomic locations.
  • Current limitations hinder understanding of enhancer function in vivo.

Purpose of the Study:

  • To review recent advancements in identifying and analyzing gene enhancers.
  • To highlight the application of CRISPR-based tools for studying enhancers in their genomic context.
  • To discuss methods for perturbing and testing enhancer activity.

Main Methods:

  • Utilizing CRISPR/Cas9 for genome editing in higher organisms.
  • Employing CRISPR-based tools for scanning noncoding genomic regions.
  • Implementing mutation and epigenetic modulation techniques.

Main Results:

  • CRISPR technology facilitates in-context analysis of enhancers.
  • Genome editing enables precise perturbation of enhancer elements.
  • New tools allow for systematic scanning of noncoding DNA for enhancer activity.

Conclusions:

  • CRISPR-based approaches are revolutionizing enhancer research.
  • Studying enhancers within their genomic context is now feasible.
  • These methods offer powerful means to investigate gene regulation and development.