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Updated: Feb 16, 2026
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Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
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Larkinella knui sp. nov., isolated from soil
Jongguen Jeon1, Leonid N Ten1, Jae-Jin Lee1
1College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Summary
A novel aerobic bacterium, Larkinella knui sp. nov., was identified from South Korean soil. This Gram-negative bacterium expands the known diversity within the Larkinella genus.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Genomics
Background:
- The family Cytophagaceae encompasses diverse bacteria with various ecological roles.
- Accurate bacterial classification is crucial for understanding microbial communities and their functions.
- The genus Larkinella includes species previously isolated from different environments.
Purpose of the Study:
- To taxonomically characterize a novel bacterial isolate from a South Korean soil sample.
- To determine the phylogenetic placement of the isolate within the Cytophagaceae family.
- To propose a new species designation for the novel bacterium.
Main Methods:
- Polyphasic taxonomic approach including phenotypic characterization.
- 16S rRNA gene sequence analysis for phylogenetic inference.
- Analysis of DNA G+C content, polar lipids, quinones, and fatty acid profiles.
Main Results:
- Strain 15J6-3T6T is a Gram-stain-negative, motile, rod-shaped, aerobic bacterium.
- Phylogenetic analysis based on 16S rRNA gene sequences showed relatedness to the genus Larkinella, with highest similarity to Larkinella harenae.
- Chemotaxonomic data (polar lipids, quinones, fatty acids) and DNA G+C content (50.6 mol%) supported affiliation with the genus Larkinella.
Conclusions:
- Strain 15J6-3T6T represents a novel species within the genus Larkinella.
- The proposed name for this new species is Larkinella knui sp. nov.
- The type strain for this novel species is 15J6-3T6T (=KCTC 42998T=JCM 31989T).
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