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Updated: Feb 16, 2026

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
Published on: September 12, 2011
Elongation of Initially Non-Polar Protoplasts is Oriented by Electric Fields
Abstract:
When regenerating Mougeotia protoplasts, suspended in agar, are cultured for 20-24 h in a 7 mV/cell D.C. field, their growth axes tend to parallel the field. This may indicate either rotation of the regenerates by the field or polarization of the protoplasts and hence polarization of their microtubules which lie perpendicular to the growth axis and regulate elongation.
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