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Updated: Feb 16, 2026
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Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice
Published on: April 3, 2021
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Local Klotho Enhances Neuronal Progenitor Proliferation in the Adult Hippocampus
Felipe Salech1,2,3,4, Lorena Varela-Nallar5, Sebastián B Arredondo5
1Department of Neuroscience, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
Summary
Klotho protein promotes adult hippocampal neurogenesis (AHN) and cognitive function. This aging-related protein directly regulates neural progenitor cell proliferation in the hippocampus.
Area of Science:
- Neuroscience
- Aging Research
- Molecular Biology
Background:
- Klotho is an aging-related protein linked to cognitive performance.
- Its role in adult hippocampal neurogenesis (AHN) remains unexplored.
- Klotho influences progenitor cell proliferation in non-neuronal tissues.
Purpose of the Study:
- To investigate Klotho's role in adult hippocampal neurogenesis (AHN).
- To determine Klotho's effect on neural progenitor cell proliferation.
- To assess Klotho's impact on hippocampal-dependent memory.
Main Methods:
- Examined Klotho expression in adult mouse hippocampus via immunofluorescence and PCR.
- Evaluated AHN in klotho knock-out (KO) and mutant mice.
- Assessed proliferation of hippocampal neural progenitor cells with recombinant Klotho.
- Measured hippocampal-dependent memory using a Morris water maze.
Main Results:
- Klotho was expressed in the Dentate Gyrus granular cell layer.
- AHN increased in klotho KO mice but not in klotho KO/vitamin D-receptor mutant mice.
- Hippocampal Klotho knockdown reduced AHN; recombinant Klotho enhanced progenitor proliferation.
- Reduced hippocampal Klotho correlated with impaired memory performance.
Conclusions:
- Klotho directly regulates adult hippocampal neurogenesis (AHN).
- Klotho promotes neural progenitor proliferation, AHN, and hippocampal cognition.
- Observed increases in neurogenesis in KO mice may involve vitamin D pathways.
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