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LncRNA DANCR functions as a competing endogenous RNA to regulate RAB1A expression by sponging miR-634 in glioma

Dawei Xu1, Jian Yu2, Guojun Gao3

  • 1Department of Neurosurgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China dawei_nero007@163.com.

Bioscience Reports
|January 6, 2018
PubMed

Insights

Long noncoding RNA DANCR is elevated in glioma, promoting tumor growth and cell cycle progression. Targeting DANCR may offer a new therapeutic strategy for glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • The lncRNA differentiation antagonizing nonprotein coding RNA (DANCR) has been implicated in various solid tumors.
  • The specific role and molecular mechanisms of DANCR in glioma progression remain incompletely understood.

Purpose of the Study:

  • To investigate the expression and function of DANCR in glioma.
  • To elucidate the molecular mechanisms underlying DANCR's role in glioma.
  • To evaluate DANCR as a potential therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for DANCR expression analysis in glioma tissues and cell lines.
  • Cell proliferation assays and cell cycle analysis to determine biological functions.
  • Luciferase reporter assays, Western blotting, and RNA immunoprecipitation (RIP) to explore molecular interactions.

Main Results:

  • DANCR expression was significantly upregulated in glioma tissues and cell lines compared to normal controls.
  • Higher DANCR expression correlated with advanced tumor grade in glioma patients.
  • Inhibition of DANCR reduced glioma cell proliferation and induced G0/G1 phase arrest.
  • DANCR was found to directly interact with miR-634, leading to the inhibition of RAB1A expression.

Conclusions:

  • The DANCR/miR-634/RAB1A axis plays a critical role in glioma progression.
  • DANCR acts as an oncogenic lncRNA in glioma.
  • DANCR represents a potential therapeutic target for glioma treatment.
Keywords:
DANCRGliomaRAB1AlncRNAmiR-634

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