Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

8.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.9K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

2.7K
2.7K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

3.1K
3.1K
Confirmation Biases01:31

Confirmation Biases

8.3K
The confirmation bias is the tendency to focus on information that confirms our existing beliefs and ignore information that is inconsistent with our expectations. For example, if you think that your professor is not very nice, you notice all of the instances of rude behavior exhibited by the professor while ignoring the countless pleasant interactions he is involved in on a daily basis. Have you ever fallen prey to the confirmation bias, either as the source or target of such bias?
8.3K
Hindsight Biases01:12

Hindsight Biases

4.3K
Hindsight bias leads you to believe that the event you just experienced was predictable, even though it really wasn’t. In other words, you knew all along that things would turn out the way they did. Can you relate this to the phrase "Hindsight is 20/20" now? 
4.3K
Bias01:22

Bias

7.4K
Bias refers to any tendency that prevents a question from being considered unprejudiced. In research, bias occurs when one outcome or answer is selected or encouraged over others in sampling or testing. Bias can occur during any research phase, including study design, data collection, analysis, and publication.
In statistics, a sampling bias is created when a sample is collected from a population, and some members of the population are not as likely to be chosen as others (remember, each member...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small-molecule modulation of β-arrestins.

Nature·2026
Same author

Divergent activation of the RXFP1 relaxin receptor by protein and small molecule agonists.

bioRxiv : the preprint server for biology·2026
Same author

β-Arrestin condensates regulate G-protein-coupled receptor function.

Nature·2026
Same author

In silico discovery of nanobody binders to a G-protein coupled receptor using AlphaFold-Multimer.

Nature communications·2026
Same author

CXCR1 and CXCR2 display receptor bias for shared chemokine agonists.

Molecular pharmacology·2026
Same author

Location-biased β-arrestin conformations direct GPCR signaling.

Science signaling·2026

Related Experiment Video

Updated: Feb 16, 2026

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
11:44

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Published on: August 15, 2014

10.8K

Biased signalling: from simple switches to allosteric microprocessors.

Jeffrey S Smith1, Robert J Lefkowitz1,2,3, Sudarshan Rajagopal1,2

  • 1Department of Biochemistry, Duke University Medical Center.

Nature Reviews. Drug Discovery
|January 6, 2018
PubMed
Summary
This summary is machine-generated.

Biased ligands offer a new approach to drug development by selectively targeting G protein-coupled receptors (GPCRs) pathways. This precision medicine strategy promises increased efficacy and reduced side effects for novel therapies.

More Related Videos

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.1K
Measuring the Switch Cost of Smartphone Use While Walking
07:00

Measuring the Switch Cost of Smartphone Use While Walking

Published on: April 30, 2020

2.3K

Related Experiment Videos

Last Updated: Feb 16, 2026

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
11:44

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Published on: August 15, 2014

10.8K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.1K
Measuring the Switch Cost of Smartphone Use While Walking
07:00

Measuring the Switch Cost of Smartphone Use While Walking

Published on: April 30, 2020

2.3K

Area of Science:

  • Pharmacology
  • Molecular Biology
  • Drug Discovery

Background:

  • G protein-coupled receptors (GPCRs) are a major class of human receptors and crucial drug targets.
  • GPCRs signal through various pathways, including G proteins and β-arrestins, which can be modulated by biased ligands.

Purpose of the Study:

  • To explore the concept of biased signaling in GPCRs.
  • To highlight the importance of evaluating biased signaling for drug development.
  • To discuss the potential of biased ligands in creating more effective and safer therapeutics.

Main Methods:

  • Review of current literature on GPCR signaling.
  • Analysis of structural studies on GPCR-transducer complexes.
  • Pharmacological and physiological evaluation of biased signaling.

Main Results:

  • GPCRs function as multistate allosteric microprocessors, not simple on-off switches.
  • Biased ligands induce preferential signaling through specific pathways (G proteins or β-arrestins).
  • Distinct signaling pathways have different physiological actions, crucial for drug development.

Conclusions:

  • Understanding GPCR biased signaling is vital for preclinical drug development.
  • Structural insights facilitate the design of novel biased therapies.
  • Biased ligands represent a promising therapeutic strategy for enhanced efficacy and reduced side effects.